CHARACTERIZATION OF THE FLUORESCENCE QUENCHING OF CHLOROPHYLL a BY 1, 4 BENZOQUINONE USING THE NONLINEAR ANALYSIS
نویسنده
چکیده
It has long been known that quinones quench chlorophyll fluorescence. This study reports the parameters of the fluorescence quenching of chlorophyll a by 1,4-benzoquinone, in ethanol, using the nonlinear analysis. The fluorescence intensity was measured at 670 nm for the blue and red excitation wavelengths: 430 nm and 650 nm, respectively. The quinone concentration in the samples ranged from 3.3×10 M to 10 M. The modified Stern-Volmer plots of the quenching data have revealed two ways of the quenching mechanism and have offered the posibility to obtain the quenching parameters.
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